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Injection Chambers (Florey), Mid 20th Century

preview image for Injection Chambers (Florey), Mid 20th Century
Inventory Number: 39420
Object Type:
Accession Number: 1973-26/10
Brief Description: 1. a single injection chamber with removable central Perspex plug.
2. Screw-on base for #1. the central plug and the 3 lock-nuts had to be removed to put the injector base on. It was fixed on with slotted hexagon slotted #3, screwed on with the special screwdriver, #4.
5. Hypodermic needle to fit channel in #2, with copper alloy adaptor to take a rubber tube leading to a micro syringe.
6. Chamber with 2 injection holes. The injection of dye was made into one hole and excess fluid drained from the other hole.
7. Screw-on base for #6. Hypodermic needle in place. fitted on like #2
8. A double injector chamber with tall buffers to get extra thick tissue.
9. Single injector base with built-in channel for dye-hole at side of the table. central plug for insertion of graft beforehand.
10. Base like #9, but position of dye-hole and graft-hole reversed.
11. set of Perspex plugs for #12, below.
12, 13, 14, 15. Parts of a "lacune" chamber. #12 is the base, put in the usual way with an 8mm central round hole. The retaining ring #13 was then put on the other side and subsequently removed. The 1st lid #14, with central rectangular protrusion was put on so that the protrusion was in contact with the base and covered the 3 holes. There were therefore 2 semi-circular spaces beside it. These soon became vascularised. When this had occurred lid #14 was removed and plain lid #15 put on in its place, thus leaving an empty space (lacune) into which a graft of lymphoid tissue was inserted through the central hole. The remaining space soon filled with exudate from the surrounding tissue. Vital dyes were injected through the oblique lateral holes.
16, 17 & 18. Chamber similar to #12 but without the central hole.
19. Base of a chamber designed to find the best thickness for a "lacune" into which cells migrated. Base inserted with the longest plug, no .4 in #20, in contact with the lid. After vascularization plug 4 was replaced by plug 3, leaving a space free from tissue 0.5mm deep. Cells migrated into this. Many moved along the coverslip. The silver pins were removed with the copper alloy tool #22 and one was replaced with the silver pin in #21 with hypodermic needle attached for the injection of vital dyes. The best thickness was found to be 0.5mm.
23. Copper alloy tool to remove Perspex plugs from chambers #2, #11 & #17.
Provenance: Apparatus of Lord Florey (1898-1968)
Collection Group: Florey Collection
Material(s): Copper Alloy
Height Width Depth Diameter Unit
268 228 20 mm

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